Determination of oxidant resistance levels of <i>P</i>. <i>aeruginosa</i> strains.

<p>The oxidant resistance levels in <i>P</i>. <i>aeruginosa</i> PAO1 and <i>fprB</i> mutants that harbored either empty vector (pBBR), pFprB (for <i>fprB</i> expression), or pISC (for expression of the functional <i>isc</i> operon without <i>iscR</i>). The resistance levels against NaOCl (0.045%) and NaNO₂ (pH 5.0, 50 μM) were determined using a bacterial killing assay while those against other oxidants i.e., H₂O₂ (0.5 mM), cumene hydroperoxide (CHP, 1.6 M), diamide (DM, 13 mM), paraquat (PQ, 200 μM), menadione (MD, 4 mM), and plumbagin (PB, 1 mM) were performed using a plate sensitivity assay as described in the experimental procedures. The data shown are means and SD of percent survival at 18 h incubation from three independent experiments. The asterisk indicates a statistically significant difference (P < 0.01) between the two indicated strains.