Real-time quantitative RT-PCR determination of SREBP-1 and SREBP-2 mRNA levels in SM-enriched adipocytes or unmodulated adipocytes.

<p>^a The control group was expressed as 1. <i>P</i><0.0001, <i>P</i><0.001, <i>P</i><0.01 and <i>P</i><0.05; SM-, GSH-, and PPMP-treated cells compared with untreated cells.</p><p>3T3-F442A adipocytes were treated with 15 μM natural SM (SM-LA, 24 h), 10 mM GSH (24 h), 20 μM PPMP (24 h) or vehicle. The mRNA levels of the studied genes were determined. The results are expressed as-fold variations over respective controls (R) after normalization to β-actin, as indicated in the Materials and methods. R values superior or equal to 2 were considered positive regulation of gene expression, whereas values lower than 0.5 indicated negative regulation. The results presented are the means of 3 independent experiments, which were performed twice each in duplicate.