mTORC1 is activated during preosteoblast proliferation but is suppressed during their differentiation.
- Bin Huang (331329)
- Yongkui Wang (308206)
- Wenhao Wang (515852)
- Juan Chen (61123)
- Pinglin Lai (779683)
- Zhongyu Liu (612014)
- Bo Yan (57900)
- Song Xu (187853)
- Zhongmin Zhang (779684)
- Chun Zeng (181389)
- Limin Rong (710428)
- Bin Liu (5899)
- Daozhang Cai (5664670)
- Dadi Jin (779685)
- Xiaochun Bai (262474)
Publication date
August 2015
DOIAbstract
<p>(A) Growth curve of proliferating MC3T3-E1 cells treated with vehicle (Veh) or rapamycin (0.1nM). (B) Western blot analysis of proliferative markers (cyclin D1 and PCNA), Osx and mTORC1 activity (indicated by P-S6K (T389) and P-S6 (S235/236)) during MC3T3-E1 proliferation. (C) Western blot analysis of osteoblastic markers (Osx and Ocn) and mTORC1 activity during differentiation of MC3T3-E1 cells. (D) Western blot analysis of osteoblastic markers (Osx and Ocn) and mTORC1 activity during differentiation of FRC. Osx: osterix; Ocn: osteocalcin; FRC: fetal rat calvarial cells, OGI: osteogenic induction.</p
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