Transcription elongation is affected by substitutions in the nucleosome acidic patch.

<p><b>(A)</b> Diagram of experimental procedure. Cells were grown in medium containing 2% galactose (zero time point) and then 2% glucose was added to shut off transcription. Samples were taken at zero, two, four, and eight-minute time points for cross-linking. ChIP of the Rpb3 subunit of Pol II across <i>YLR454W</i> was performed in <b>(B)</b> wild type (WT), <b>(C)</b> H2A-E65A, <b>(D)</b> H2A-L66A, <b>(E)</b> H2A-E93A, and <b>(F)</b> H2B-K123R strains, which were transformants of KY2676. Values were normalized to the zero time point for each locus. Error bars represent SEM of three biological replicates.