Additional file 5: of MiR-106b induces cell radioresistance via the PTEN/PI3K/AKT pathways and p21 in colorectal cancer

MiR-106b induces CRC cell resistance to irradiation. Figure S1. (A) The expression pattern of miR-106b in CRC cell lines with different degrees of differentiation. The relative expression of miR-106b was normalized to the endogenous control U6. (B) The up-regulation or inhibition of miR-106b in SW620 via lentiviral transduction in SW480 cells was detected using qRT-PCR. *p<0.05. (C) γ-H2AX was examined by immunofluorescence in the HT-29 cells transfected with miR-106b when exposed to radiation (4 Gy, 6 h). The γ-H2AX staining is shown in the left panels, and the numbers ofγ-H2AX foci are shown in the right panels. *p<0.05. (D) Apoptosis as evaluated by flow cytometry when exposed to radiation (4 Gy). **P<0.005, **P