SRPK2/SRPIN340 interaction studied by fluorescence spectroscopy.
- Raoni Pais Siqueira (779962)
- Éverton de Almeida Alves Barbosa (779963)
- Marcelo Depólo Polêto (779964)
- Germanna Lima Righetto (779965)
- Thiago Vargas Seraphim (238321)
- Rafael Locatelli Salgado (779966)
- Joana Gasperazzo Ferreira (422933)
- Marcus Vinícius de Andrade Barros (779967)
- Leandro Licursi de Oliveira (374413)
- Angelo Brunelli Albertoni Laranjeira (753639)
- Márcia Rogéria Almeida (660053)
- Abelardo Silva Júnior (779968)
- Juliana Lopes Rangel Fietto (133977)
- Jörg Kobarg (228176)
- Eduardo Basílio de Oliveira (779969)
- Robson Ricardo Teixeira (779970)
- Júlio César Borges (779971)
- Jose Andrés Yunes (5664634)
- Gustavo Costa Bressan (660052)
Publication date
August 2015
DOIAbstract
<p>(A) DMSO titration (0–5% v/v) of SRPK2 spectra showing no significant changes in the local tertiary structure of the W residues. (B) Fluorescence spectra of the SRPK2 titration with increasing concentrations (0–15 μM) of SRPIN340. (C) Data analysis of the spectra shown in (B) and SPRK2/SRPIN340 dissociation constant (K<sub>D</sub>) determination. The K<sub>D</sub> found for this interaction was 1.6 ± 0.3 μM. (D) SRPK2 (1 μM) fluorescence spectra upon addition of several concentrations of ATP analogue.</p
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