CD spectra of Hb A, rHb(αH87G) and rHb(βH92G).

<p>Spectra are Hb A (A), rHb(αH87G) (B) and rHb(βH92G) (C) in the deoxy (blue spectra) and oxy forms (red spectra) in the wavelength region between 275 nm and 320 nm. Hemoglobin concentration, 45 μM (in heme) in a 0.05 M phosphate buffer (pH 7) containing 5 mM imidazole and a metHb reducing system. The broken black lines in (B) and (C) indicate the curve of deoxy Hb A.