Changes in soil bacteria community structure.

<p>(a) Bacterial colony-forming units assessed using the culture plating method. All values are the means ± SD (n = 3). (b) Similarity dendrogram of the bacterial banding patterns of soil samples. The scale for 0.44–1.00 was the similarity coefficient among the samples. DGGE fingerprints of 16S rDNA sequences amplified using the primer pair 357fGC/518r from soil-extracted community DNA. Lanes 1, 2 and 3 show the results of soil samples without salecan treatment (C); lanes 4, 5 and 6 show the results of 0.02% salecan-treated samples; and lanes 7, 8 and 9 show the results of 0.2% salecan-treated samples. Seven changed DGGE bands (B1, B2, B3, B4, B5, B6, B7) are marked by arrows. (c) The phylogenetic relationship between the 16S rRNA gene sequences of the soil samples and the related organisms from the GenBank database. The dendrogram was generated using the neighbor-joining method, with 1000 bootstrap resamplings. The scale bar represented 0.02, estimated nucleotide changes per sequence position.