<i>mrg-1</i> (<i>lf</i>) exhibits increased failure to form bivalents when combined with meiotic checkpoint mutations

<p>(A) Full projections of optically sectioned DAPI-stained DNA images in oocytes from positions -1 to -3. The periphery of each oocyte is indicated by the dotted circle. Six DAPI-stained bodies per oocyte were usually observed in the <i>mrg-1</i> single mutant, demonstrating successful bivalent formation. The <i>cep-1; mrg-1</i> or <i>pch-2; mrg-1</i> double mutants frequently displayed diakinesis oocytes with more than six DAPI-stained bodies, indicating failed bivalent formation. The <i>cep-1; pch-2; mrg-1</i> triple mutant showed the most obvious failure in bivalent formation among the four strains tested. All four strains have ced-1::GFP in the background (not shown). The same image data that was analyzed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134871#pone.0134871.g003" target="_blank">Fig 3</a> was used for this analysis. Bar: 10 μm. (B) Histogram showing the percentage of gonads with oocytes from the -1 to -3 positions displaying more than six DAPI-stained bodies. The <i>cep-1; mrg-1</i>, <i>pch-2; mrg-1</i>, and <i>cep-1; pch-2; mrg-1</i> mutants display substantially increased populations of oocytes with more than six DAPI-stained bodies in at least one of three late diakinesis oocytes. This increase is statistically significant except for <i>pch-2; mrg-1</i> when compared to the <i>mrg-1</i> single mutant (p = 0.0374, p = 0.0738, p = 0.0015, respectively, Fisher’s exact test).