Brain PrP^Sc in ovine PrP transgenic rabbit infected with LA21K fast scrapie prions.

<p>(a) Western blot analyses of the brain from wild-type and tgOv rabbits mock-infected or inoculated with LA21K <i>fast</i> prions for the presence of proteinase K (PK)-resistant PrP^Sc. The equivalent of 2 mg brain tissue were loaded in lane 1–6, 3 mg in lanes 7 and 8. (b) Electrophoretic pattern and (c) glycoform ratios (plotted as means ± SEM(c)) of PrP^res in the brains and spleens of tgOv rabbit and tg338 mice infected with LA21K fast prions. The same amount of brain (0.5 mg) and spleen (3 mg) tissue equivalent was loaded on the gel.