Representative flow cytometry profiles and comparative binding selectivity of the uptake of SA43 and SA44 DNA on various cell lines.

<p>Cy3 labelled SA43, SA44 and random aptamer (100 nM) were incubated separately on live U87MG, 1321N1, SVGp12, T24 and MCF-7 cells at 37°C (5% CO₂) for 90 minutes. Untreated cells were incubated with complete growth medium alone. The blue curve represents the background mean fluorescence intensity (MFI) of the untreated cells (control), and the green and red curve represents the MFI (uptake) of the cells treated with random aptamer and the lead aptamers respectively. <b>A</b>, <b>B</b>, <b>C</b>, <b>D</b>, and <b>E</b> represent uptake of SA43 on U87MG, 1321N1, SVGp12, T24 and MCF-7 cells respectively. <b>G</b>, <b>H</b>, <b>I</b>, <b>J</b> and <b>K</b> represent uptake of SA44 on U87MG, 1321N1, SVGp12, T24 and MCF-7 cells respectively. <b>F</b> and <b>L</b> are bar charts showing comparative binding selectivity of aptamer SA43 and SA44 across various cell types. The background MFI values for the random aptamer treated cells were subtracted. Statistical significance of the differences in MFI values of SA43 and SA44 between U87MG and 1321N1 versus SVGp12, T24, and MCF-7 confirm high binding selectivity of the aptamers for U87MG and 1321N1 cells (** = <i>p</i> < 0.01). No statistical difference was observed between U87MG and 1321N1 cells for both the aptamers (<i>p</i> > 0.05; n = 3).