Inhibition of β-catenin expression by embelin.

<p>(A) Whole cell lysates were prepared and extracted proteins were resolved by SDS-PAGE (10%) and Western blot analysis using β-catenin, phospho-β-catenin, or GSK-3β antibodies was conducted. The numbers between the blots are the ratios of the intensity of bands after normalized with control. (B) Luciferase assay. TOPFlash or FOPFlash-transfected cells were treated with embelin. The luciferase activity was measured and the relative firefly luciferase activity, normalized by the renilla luciferase activity, is shown. (C) Cells were seeded on cover slip for 24 h and treated with 30 μM embelin up to 24 h. Then the cells were stained with β-catenin antibody and fluorescence was determined using confocal microscopy. (D) Expression of cyclin D1, c-Myc and MMP-7 was determined by using quantitative PCR and Western blot analysis. Values represent mean ± S.D. of three independent determinations.<b>*,</b>^<b>#</b><b>,</b>^<b>§</b>, significantly different from the untreated control (<i>p</i>< 0.05). The numbers between the blots are the ratios of the intensity of bands after normalized with control.