Efficient DT-induced pDC depletion reduces macrophage and neutrophil numbers in lungs and blood of <i>Apoe</i>^<i>-/-</i><i>BDCA2-DTR</i> mice after intravenous LPS administration in an iALI model.

<p>(A) H&E stained histological lung sections of <i>Apoe</i>^<i>-/-</i><i>BDCA2-DTR</i> mice after 24 hours after DT administration and 12 hours after i.v. LPS administration (100 μg/mouse) to iALI, (10x magnification and scale bars = 50 μm). (B) Flow cytometry analysis of CD45⁺Gr1⁺CD115^- neutrophils and F4/80⁺ macrophages in lungs (cells/lung) and (C) of CD45⁺Gr1⁺CD115^- neutrophils and CD45⁺CD11b⁺Gr1^-CD115⁺ monocytes in blood (cells/ml) after iALI in pDC depleted <i>Apoe</i>^<i>-/-</i><i>BDCA2-DTR</i> mice. (D) Plasma cytokine levels of Il-6, Cxcl1 and Tnf-α (pg/ml). Graphs represent mean±SD; n = 9 to n = 16; Mann-Whitney test or unpaired t-test with Welch‘s correction *<i>P</i><0.05 **<i>P</i><0.01 ***<i>P</i><0.001.