EV71 infectivity after NE treatment on induced THP-1.

<p>(A) PMA-induced THP-1 were infected with EV71 (MOI = 10) for 24 hrs and then treated with NE for 24 hrs. The RD cells were infected with EV71 (MOI = 10) for 24 hrs as positive control in each infection replica. Mock-infected cells were used as negative control to gate infected cells. The percentages of EV71-infected THP-1 were increased after NE treatment. (B) Viral particles (green fluorescence) were observed on NE treated and EV71-infected THP-1 using immunofluorescent microscopy. Mock-infected and non-NE-treated THP-1 and secondary antibody stained THP-1 were performed as negative controls. DAPI (blue) staining indicated cell nucleus. The data were represented from three independent experiments.