Effects of INO-1001 treatment on striatal neuron number in R6/2 mice.

<p>Confocal laser scanning microscopy images of single-label immunofluorescence for the marker of the medium spiny neurons, Calbindin D-28K (green), in the striatum of a vehicle treated wild type (<b>A</b>), INO-1001 treated wild-type (<b>B</b>), vehicle treated R6/2(<b>C</b>), or R6/2 mouse treated with INO-1001 from 5 weeks of age (<b>D</b>). (<b>E</b>) Quantification of the number of striatal neurons labeled with CALB. A two way ANOVA indicated a significant effect of genotype (F(1,72) = 12,18474; p = 0,0008), treatment (F(1,72) = 5,442040; p = 0,0225), and genotype X treatment interaction (F(1,72) = 5,019067; p = 0,0282). Data were analyzed with GraphPad Prism statistical software 6.0e using two-way ANOVA followed by a Tukey’s multiple comparison test. A Tukey’s multiple comparison test indicated that the density of striatal neurons in R6/2 mice treated with INO-1001 was significantly greater than that of vehicle treated R6/2 animals (<i>P</i><0.001).