Delivery of pICLC into the airways induces the rapid influx of neutrophils and Ly6C^high monocytes into the lung tissue parenchyma and suppresses <i>C</i>. <i>neoformans</i> induced eosinophilia.

<p>(<b>A</b>) Gating strategy used in these experiments to identify distinct myeloid cell subsets. Example plots are taken from the lungs of a naïve animal. (<b>B-D</b>) Naïve and H99 infected mice were intrapharyngeally administered PBS or pICLC once at time of infection and myeloid cell responses were analyzed on day 3. To discriminate intravascular and parenchymal leukocytes, mice were intravenously injected with anti-CD45.2-FITC and euthanized 3 minutes later. (<b>B</b>) Example FACS plots of the intravascular stain after gating on Ly6C^high monocytes as shown in (A). Percentage (<b>C</b>) and absolute number (<b>D</b>) of each myeloid cell type that is intravascular stain negative in the lung. Data in (<b>B-D</b>) on day 3 post-infection are pooled from 2 separate experiments with n = 5 mice/group. (<b>E</b>) The percentage of total live lung cells that are each myeloid cell type in untreated and pICLC treated mice on day 20 post-infection. (<b>F</b>) Number of each myeloid cell type in the lungs that is intravascular stain negative on day 20 post-infection. Data were pooled from two independent experiments with n = 4–5 mice/group.