Normal macrophage polarization in CD73-deficient animals.

<p><b>(A)</b> Gene expression (mean ± SEM) of <i>Nos2</i> (a pro-inflammatory macrophage marker) in peritoneal cells after the indicated <i>in vivo</i> polarization was determined using qPCR. <b>(B)</b> The concentrations of the cytokines IL-6, IL-12 (p40) and KC (pro-inflammatory macrophage markers) in the peritoneal lavage fluid of wild-type (WT) and CD73-deficient (KO) mice after the <i>in vivo</i> polarizations. <b>(C)</b> Gene expression (mean ± SEM) of <i>Mrc1</i> and <i>Arg1</i> (both anti-inflammatory macrophage marker) in peritoneal cells after <i>in vivo</i> polarization was determined using qPCR. <b>(D)</b> Intracellular expression of Ym-1, <b>(E)</b> intracellular expression of Relm alpha and (<b>F</b>) surface expression of CD206 (all anti-inflammatory macrophage markers) was determined on total peritoneal leukocytes using flow cytometry and is shown together with representative histograms (specific stainings in white, isotype control stainings in grey). All data are from 6–9 mice/group (except in M(-) control groups where <i>n</i> = 2–4 and in F where <i>n</i> = 3–8). In (B, D, E, F) each dot represents an individual experimental value, the vertical line the median and the whiskers the interquartile ranges. Data are from at least 4 different experiments. NA = not available.