Activation of non-canonical TGFβ pathway through p-JNK.

<p>DBA iPSCs, the corrected DBA cells and the GFP cells were cultured, and RNA, total protein and the protein from nuclear and cytoplasmic fraction were extracted from iPSCs for analysis. Fibrillarin were used as loading controls. A) Overexpression of <i>JNK1</i> mRNA level in both DBA mutant lines. B) Elevation of JNK protein, especially p-JNK, in the total protein of DBA iPSCs with <i>RPS19</i> mutation. C) Accumulation of nuclear p-JNK in the DBA iPSCs and the GFP lines (especially in <i>RPS19</i> mutant line), and restoration of p-JNK levels in the corrected DBA lines. D) Increase of cytoplasmic p-JNK in the DBA iPSCs, and normal levels of p-JNK in the rescued DBA lines.