The anti-proliferative effects of GCs on naïve ADSCs are reversed by sodium orthovanadate (SOV).

<p>(a-d): Naïve ADSCs were plated into 24-well plates and grown to 80% confluence, after which they were cultured in DMEM + 1% FBS for 24h. (a,b): Cells were subsequently treated for 24h with (a) DMEM + 1% FBS or (b) DMEM + 5% FBS, in the absence (EtOH) or presence of different concentrations of dexamethasone (Dex). (c,d): Cells were subsequently treated for 24h with (c) DMEM + 1% FBS or (d) DMEM + 5% FBS in the absence or presence of 5 nM Dex and 1 μM SOV. For a-d, proliferation was quantified by means of [³H]dT incorporation. All panels show the representative results of three independent experiments, performed in triplicate. Different lower-case letters indicate statistically significant differences (P < 0.05).