Cultivation and evaluation of eGFP expression using the BioLector system.

<p>a) Cultivation in the BioLector showing stable temperature at 27°C, humidity at around 85%, growing cell density and increasing eGFP fluorescence for Hi5 cells transfected with the OpIE2-eGFP plasmid b) Correlation between cell density measured in BioLector and cell number measured in Guava c) Transfection efficiency measured in the Guava 52 h after transfection. A transfection efficiency of 20% was reached in this experiment.