Enh A, ISRE and X-box are important elements for the endogenous and KA induced MHC-I promoter activity in neuro2a cell lines.

<p>(A) Schematic illustration of constructs used for HLA-A heavy chain promoter-luciferase reporter assay. (B) At 6, 8 and 10 hours post 100 μM KA treatment for 30min increased the expression level of H-2K mRNA and protein (C). (D) Luciferase activity of different promoter-reporter plasmids in neuro2a cell lines. Mean luciferase activity was calculated from three independent experiments and shown with the SD. (E) Relative luciferase activity of different promoter-reporter plasmids in neuro2a cell lines by using 100μM KA for 30min.