Morphology of MCF-7 cells grown in 2D and 3D cultures.

<p>MCF-7 human breast cancer cells grown in 2D monolayer culture for 3 days on poly-L-lysine coated coverslips and stained with periodic acid-Schiffs and hematoxylin (PASH) under low (A) and high (B) magnification. Cells exhibit typical cobblestone morphology, with several aggregates of cells (asterisks). MCF-7 human breast cancer cells grown in scaffold free non-adhesive agarose hydrogels for 7 days form microtissues with luminal spaces containing PAS-positive secretions (arrows) under low (C) and high (D) magnification. Scale bar = 50μm.