Mitochondrial dysfunction in <i>fahd-1(tm5005) C</i>. <i>elegans</i>.

<p>(A) 3-day old <i>fahd-1(tm5005)</i> and wild-type <i>C</i>. <i>elegans</i> were incubated with 100 nM TMRE overnight. <i>Left panel</i>: <i>fahd-1(tm5005)</i> animals exhibit a decreased mitochondrial membrane potential as shown by fluorescence microscopy. Representative images are shown. Size bar = 100 μm. <i>Right panel</i>: For quantification of the pixel intensities, fluorescence pictures were analyzed using ImageJ (25.93 ±SD 7.61 for wt vs. 17.69 ±SD 9.87 for <i>fahd-1(tm5005)</i>; p = 0.0005; N = 30). (B) Oxygen consumption rate was determined in wild-type and <i>fahd-1(tm5005)</i> animals by Oxygraph 2K measurement and the basic respiration rate was determined. In <i>fahd-1(tm5005)</i> animals this parameter is significantly reduced (0.0659 ±SD 0.0026 pmol sec^-1 worm^-1 for wt vs. 0.0.0571 ±SD 0.0062 pmol sec^-1 worm^-1 for <i>fahd-1(tm5005)</i>; p = 0.0475; N = 4).