HMB induced neurite outgrowth is mediated by PI3K/Akt and ERK1/2 signaling pathways in Neuro2a cells.

<p>Neuro2a cells were treated with 25 μM HMB for 30 min. Western blot analysis was performed using specific antibodies against phospho- and total-antibodies against Akt <b>(A)</b> and ERK1/2 <b>(B)</b>. <b>(C)</b> Neuro2a cells were pre-treated with LY294002 20μM or PD98059 10μM and then treated with 25 μM HMB for 48 h. Inhibitors were maintained during the experiment. Cell morphology was measured as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135614#pone.0135614.g001" target="_blank">Fig 1</a>. Results represent means ± SEM (n = 4). * p<0.05 versus control cells. # p<0.05 versus HMB treated cells.