Effects of ethanol on gene expression in cortical neurons differentiated from H1 hESCs.

<p>Expression changes of nine genes in H1 hESC differentiated cortical neurons exposed to 50 mM ethanol for 7 days plus 24-hr ethanol withdrawal treatment were determined by RT-qPCR. Left column for each gene: without ethanol exposure; right column for each gene: with ethanol exposure. The expression level of each gene in ethanol-unexposed neurons was normalized to “1”. <i>GRIN1</i>, <i>2A</i>, <i>2B</i>, and <i>2D</i>: the NMDA receptor subunit genes; <i>BCL2</i>: the B-cell CLL/lymphoma 2 gene; <i>CCND2</i>: the cyclin D2 gene; <i>ALDH2</i>: the aldehyde dehydrogenase 2 gene; <i>ITPR2</i>: the inositol 1,4,5-trisphosphate receptor (type 2) gene; and <i>JARID2</i>: the jumonji- and AT-rich interactive domain 2 gene. The results were representative of three separate experiments. Each value represented the mean ± SD of triplicate wells.