Cell wall damage followed by remodeling occurs during adaptation to 5-flucytosine stress.

<p>A) <i>C</i>. <i>glabrata</i> KUE100 cells exposed to lyticase stress were harvested in the exponential phase of growth in the absence of stress (♦) or upon 30 min of exposure to 0.7 mg/L 5-flucytosine (■), or in the exponential phase of growth reached upon adaptation to 0.7 mg/L 5-flucytosine (▲). The different cell populations were washed with water and resuspended in 0.1M sodium phosphate buffer at pH 7.5. After addition of 20mg/L lyticase, the decrease in the OD_600nm of the cell suspension was measured periodically and indicated as a percentage of the initial OD_600nm. The indicated values are averages of at least three independent experiments. Error bars represent the corresponding standard deviation. * P<0.05; ***P<0.001. B) Comparison, by spot assays, of the susceptibility of <i>C</i>. <i>glabrata</i> KUE100 cells harvested in the exponential phase of growth in the absence of stress (control) or upon 30 min of exposure to 0.5 mg/L 5-flucytosine (30’ 5-FC), or in the exponential phase of growth reached upon adaptation to 0.5 mg/L 5-flucytosine (exp 5-FC) to Calcofluor White, in MMB agar plates. The inocula were prepared as described in Materials and Methods, and the cell suspensions used to prepare the spots 1:5 (b) and 1:25 (c) dilutions of the cell suspension used for column a. The images are representative of at least three independent experiments.