Extrusion of Chromatin in Cultured MTFs.

<p>Representative 3D surface rendered confocal images of cultured MTFs stained for nuclei with DAPI (Blue), showing extrusion of DNA from nuclei. There was apparent “Shedding” of DNA from the nuclei into the cytoplasm, which was evident in essentially all of the cultured MTFs. In many cases, this appeared as tubes of chromatin being extruded, in other cases as sheets. Panc-1 cells showed a reduced extrusion of DNA, which appeared much finer than that seen in CTCs.