<i>PTPRD</i> mutation is associated with increased pSTAT3 (Y705) expression in HNSCC tumors and cell lines.

<p>(A) HNSCC tumors harboring <i>PTPRD</i> mutations express increased pSTAT3 (Y705) relative to <i>PTPRD</i> wild-type tumors as determined by whole exome sequencing and reverse-phase protein array (RPPA). Two-tailed unpaired t test. (B) Overexpression of wild-type PTPRD in a <i>PTPRD</i>-mutant cell line (Cal27) leads to decreased pSTAT3 (Y705) expression (C) Quantitation of three replicate experiments as shown in B. Two-tailed unpaired t test. (D) <i>PTPRD</i>-wild-type HNSCC cells (PE/CA-PJ34clone12) transiently overexpressing mutant PTPRD exhibit increased pSTAT3 (Y705) expression relative to wild-type-expressing cells. (E) Quantitation of three replicate experiments as shown in D. Two-tailed unpaired t test.