Proliferation of follicles in grafts at different time-points.

<p>(A) Immunohistochemical staining for the cell proliferation marker, PCNA, in the transplanted ovaries. Red-brown coloring of the nucleus of the granulosa cells was recorded as positive staining (bi-directional arrow). (A1) fresh control. (A2-6) ovaries after grafting (1 day, 3 days, 1 week, 2 weeks, and 1 month, respectively). Original magnification: ×400. Scale bar = 50 μm. (B) The proportion of PCNA-positive primordial, primary, secondary and antral follicles for various time-point grafts. PCNA-positive rate of primordial and primary follicles, respectively, showed significantly increased and decreased trends when grafting time increased. * means significant difference compared to the 1 week, 2 weeks and 1 month time points (<i>P</i> <0.05). * *means significant difference compared to the 1 month time point (<i>P</i> <0.05) 1D = 1 day, 3D = 3 days, 1W = 1 week, 2W = 2 weeks, 1M = 1 month.