Expression of both <i>pkdcc1</i> and <i>pkdcc2</i> is induced downstream of Wnt canonical signaling.

<p>(A-H) Axis perturbation assay. Whole-mount <i>in situ</i> hybridization of gastrula stages untreated embryos (A, E) or treated with UV (B, F) or LiCl (C, D, G, H) and hybridized with (A-D) <i>pkdcc1</i> or (E-H) <i>pkdcc2</i> probe. Embryos are shown in a vegetal view with the dorsal side at the top and ventral side at the bottom. Hemisections are displayed with the dorsal side to the right. (I) qPCR for <i>pkdcc1</i> and <i>pkdcc2</i> expression on wild type embryos and uninjected and <i>β-catenin</i> or <i>wnt8</i> injected animal caps. (J) qPCR analysis of <i>xnr3</i> and <i>siamois</i> (<i>sia</i>) expression on wild type embryos and uninjected and <i>pkdcc1</i> or <i>pkdcc2</i> injected animal caps. D, dorsal; V, ventral. (p<0.05, in the Student’s <i>t</i>-test).