Suppression of local GFP silencing in <i>N</i>. <i>benthamiana</i> leaves.

<p>(A) Fluorescence images (5 dpi) from leaves co-infiltrated with pBinGFP and NSs gene constructs (TSWV, GRSV, TYRV) in binary vector pK2GW7. A leaf infiltrated with pBinGUS was included as control. (B) Number of fluorescence units [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134517#pone.0134517.ref028" target="_blank">28</a>] measured in leaf disks (1 cm²) collected from the agroinfiltrated leaf areas shown in panel A. Error bars indicate standard error of the mean (SEM) of three replicates. (C) Western immunoblot detection of TSWV and GRSV NSs (using antibody specific for TSWV NSs), and their corresponding his-tagged constructs, transiently expressed in <i>N</i>. <i>benthamiana</i>. A non-specific band (*) was used as loading control. (D) Western immunoblot detection of his-tagged constructs from TSWV, GRSV and TYRV NSs (same samples as panel C) using anti-polyhistidine antibody (Sigma Aldrich). Marker sizes are indicated at the right hand side.