Systemic GFP silencing 17 days after agroinfiltration of leaves L3 and L4 with pBinGFP and pBinGUS.

<p>(A) Systemic GFP silencing in a <i>N</i>. <i>benthamiana</i> 16C plant, showing vertical leaf positions. White letters indicate the position of leaves analysed for systemic silencing. Agroinfiltrated leaves L3 and L4 are not visible, and their positions are indicated (yellow letters). (B) Visual overview on systemic GFP silencing in leaves L5—L10 from ten <i>N</i>. <i>benthamiana</i> 16C plants. (C) Visual Systemic Silencing Index (VSSI) of systemic GFP silencing in leaves shown in panel B. (D) Digital Systemic Silencing Index (DSSI) of systemic GFP silencing in leaves shown in panel B. (E) Angular leaf position (0–180 degrees) of leaves L7—L10 and the agroinfiltrated leaf L3 relative to the agroinfiltrated leaf L4 (set as reference on zero degrees). Error bars in panels C and D indicate the standard error of the mean (SEM) of measures resulting from 10 leaves.