Detection of CASPASE-3 in cumulus cells by immunofluorescence after COCs were cultured with acrylamide for 14h.

<p>(A) COCs representative samples from the control group (a1, a2 and a3), the 5 μM acrylamide-treated group (b1, b2 and b3), the 10 μM acrylamide-treated group (c1, c2 and c3), and the 20 μM acrylamide-treated group (d1, d2 and d3) are shown. Green staining indicates CASPASE-3 expression in cumulus cells (a1, b1, c1, d1), cell nucleus are stained blue (a2, b2, c2, d2), a3, b3, c3, d3 is the merge of (a1, a2), (b1, b2), (c1, c2) and (d1, d2), respectively. Bar = 25 μm. (B) Positive cumulus cell ratio of CASPASE-3 protein in 0, 5, 10 and 20 μM ACR-treated group. The number of positive cumulus cell was counted and the ratio to the number of nuclei was analyzed for each group. Data is expressed as the mean ± SD of three separate experiments. * indicates <i>P</i> < 0.05 vs. control, ** indicates <i>P</i> < 0.01 vs. Control.