Effect of ACR on the maturation of mouse oocytes (COCs) <i>in vitro</i>.

<p>(A) COCs morphology before culturing (a1, b1, c1, d1) and the polar body I (PB I) of oocyte which cumulus cells were removed after COCs cultured for 14h treated with acrylamide of 0 μM (a2), 5 μM (b2), 10 μM (c2) and 20 μM (d2); Bar = 150 μm. (B) The percentage of GVBD and PB I after COCs cultured for 14h treated with experimental dosage of acrylamide. The data was compared using chi-square test and <i>t</i>-tests. Data is expressed as the mean ± SD of three separate experiments. * indicates <i>P</i> < 0.05 vs. control, ** indicates <i>P</i><0.01 vs. Control. 0 μM group: n = 147; 5 μM group: n = 164; 10 μM group: n = 172; 20 μM group: n = 168.