CXCR4 expression and desensitization are independent of SOCS3 signaling in B-lineage cells.

<p><b>A</b>, CXCR4 expression in developing B cell subsets in BM of <i>Socs3</i>^<i>fl/fl</i> (red) or <i>Socs3</i>^<i>+/+</i> (black) <i>Mb1</i>^<i>Cre/+</i> mice. <b>B</b>, Mean fluorescence intensity (MFI) of CXCR4 expression on the surface of developing B cell subsets isolated from BM of <i>Socs3</i>^<i>fl/fl</i> (red) or <i>Socs3</i>^<i>+/+</i> (white) <i>Mb1</i>^<i>Cre/+</i> mice. Bars indicate the mean, circles depict individual mice analyzed. Data was pooled from 3 independent experiments. <b>C</b>, Fold change in CXCR4 expression in IgM⁺IgD^lo cells distributed in BM parenchyma and sinusoids. White, <i>Socs3</i>^<i>+/+</i><i>Mb1</i>^<i>Cre/+</i>; Red, <i>Socs3</i>^<i>fl/fl</i><i>Mb1</i>^<i>Cre/+</i> mice. Bars indicate average and standard deviation. <b>D</b>, Change in MFI of CXCR4 surface expression on B lineage cell subsets from <i>Socs3</i>^<i>+/+</i><i>Mb1</i>^<i>Cre/+</i> (white) or from <i>Socs3</i>^<i>fl/fl</i><i>Mb1</i>^<i>Cre/+</i> (red) mice stimulated with CXCL12 at the indicated concentrations. Data shown is the ratio between CXCR4 MFI in unstimulated cells and after stimulation. Bars indicate the mean, circles depict individual mice analyzed. Data was pooled from 3 independent experiments.