Nuclear localization of GNL3L promotes ‘S’ phase progression.

<p>HEK293 (<b>A</b>) and MCF-7 (<b>B</b>) cells were transfected with wild type or indicated variants of GNL3L and the asynchronous cell cycle profiles were analyzed. (<b>C</b>) MCF-7 cells transfected with wild type or variants of GNL3L were synchronized by single thymidine block as described in Materials and Methods. Cell cycle profiles were analyzed at 0, 4, 6 and 9 h post thymidine release by flow cytometry. Propidium Iodide (PI) was used to label the nuclei. (<b>D</b>) HEK293 cells were transfected with wild type or indicated variants of GNL3L and the DNA synthesis was measured by incubating the cells with 1X BrdU for 4 h followed by staining with anti-BrdU primary antibody and HRP-conjugated secondary antibody. Stained cells were incubated with TMB substrate and the absorbance was read at 450 nm.