GMF induces CCL2 release from mouse primary mast cells.

<p>In dose-response and time-course studies, BMMCs were incubated with GMF and assayed CCL2 level in the culture supernatant media by ELISA (n = 4). (A) GMF significantly induced CCL2 release from BMMCs-derived from wild type mice. Further, BMMCs derived from GMF-KO mice (B) released less CCL2 when compared to CCL2 released from BBMCs (A) derived from wild type mice. The control cells were treated with equal volume of medium containing 0.1% BSA PBS. Results were presented as mean ± SEM (*p<0.05 control vs GMF treated, ANOVA and Tukey-Kramer).