Influence of CycG on Akt1-Wdb binding.

<p>(A) Akt1 and Wdb proteins interact <i>in vivo</i> in <i>cycG</i>^<i>HR7</i> mutants but not in the wild type. Akt1 proteins were immunoprecipitated (IP) from head extracts of either wild type or <i>cycG</i>^<i>HR7</i> mutant animals and probed with anti-Akt1 (left panels, arrows) or anti-Wdb antibodies (right panels, arrows) as indicated. The input lane contained 25% of the protein extract (PE) used for the IP. Despite low Akt1 abundance in head extracts, Akt1 was robustly immunoprecipitated in wild type or <i>cycG</i>^<i>HR7</i> mutants (left panels, open arrows), whereas Wdb protein was only co-precipitated in the mutant (right panels, double headed arrows). Arrowheads point to unspecific signals. Unrelated serum was used as mock control. The asterisks label unspecific IgG signals. M, size standard in kDa. (B) Wdb proteins were detected by Western blotting in head extracts of either <i>cycG</i>^<i>HR7</i>mutants or wild type (Wt) control as indicated. Beta-Tubulin (Tub) served as loading control.