Additional file 1: of Evidence that the endosomal sorting complex required for transport-II (ESCRT-II) is required for efficient human immunodeficiency virus-1 (HIV-1) production

Figure S1. Validation of shRNA system for the study of HIV-1 replication. (A) Western blot detecting the knockdown of DDX3 by shDDX3 (target sequence CATTGAGCTTACTCGTTAT) in HeLaM cells. (B) Cell viability assay performed as described for Figure 1A. (C-D) Production of infectious pseudotyped (c, mean ± SEM, n = 4) and wild type (d, mean ± SD of duplicate samples) viruses upon knockdown of DDX3, 96 h post-transfection. Transfections and analyses were performed as described for Figure 1