The effect of different germination media on pollen germination in vitro of the walnut (Juglans regia L.) cultivar ‘Ibar’

<p>Different culture media were tested in an attempt to improve the knowledge of the most suitable germination<br>media for studying in vitro pollen germination capacity of the Serbian walnut cultivar ‘Ibar’. The research was<br>conducted on agar media, as a four-factorial experiment, with different concentrations of agar (0.6%, 0.8% and<br>1%), sucrose (10%, 15% and 20%), H3BO3 (0 ppm, 200 ppm and 400 ppm) and CaCl2 (0 and 50 ppm). Pollen<br>germination was maximized (38.5%) when the germination medium contained 0.6% of agar, 15% of sucrose,<br>200 ppm of H3BO3 and 50 ppm of CaCl2. Significant differences in pollen germination were observed in response<br>to changing concentrations of agar, sucrose, boric acid and calcium chloride. Significant interactions were<br>detected between most of these substances.