Accumulation of Tfh2 cells was associated with increased frequency of plasma cells in patients with schistosomiasis japonica.

<p>(A) Gating schemes for analysis of the percentages of different subsets of B cells. PBMCs were stained with CD3, CD4, CD19, CD27, CD86, and CD38 antibodies. Gating strategy defining memory B cells (R4), naive B cells (R5), activated B cells (R6), and plasma cells (R7); (B) Percentage of total B cells in total lymphocytes; (C-H) Representative flow cytometry data plots and statistics show the memory B cells (C, D), naïve B cells (C), activated B cells (E, F) and plasma cells (G, H); (I, J) The correlation analysis among the percentage of Tfh2 cells and activated B cells (I) as well as plasma cells (J). All flow cytometry results were analyzed and plotted using Fluorescence Minus One controls (FMO). **P<0.01. ***P<0.001.