Ampkα1 sensitive up-regulation of Tgf-β-activated protein kinase 1 (Tak1) following unilateral ureteral obstruction.

<p><b>A.</b> Representative original Western blots and arithmetic means ± SEM (n = 9) of normalized Tgf-β precursor/Gapdh protein ratio in renal tissue from non-obstructed control kidney (CTR) and obstructed kidney (UUO) of Ampkα1 knockout mice (black bars, Ampkα1^-/-) and respective wild-type mice (white bars, Ampkα1^+/+) following 7 days of unilateral ureteral obstruction. <b>B.</b> Representative original Western blots and arithmetic means ± SEM (n = 9) of normalized phospho-Tak1 (Ser⁴¹²)/ Gapdh protein ratio and total Tak1/ Gapdh protein ratio in renal tissue from non-obstructed control kidney (CTR) and obstructed kidney (UUO) of Ampkα1 knockout mice (black bars, Ampkα1^-/-) and respective wild-type mice (white bars, Ampkα1^+/+) following 7 days of unilateral ureteral obstruction. <b>C.</b> Representative original Western blots and arithmetic means ± SEM (n = 9) of normalized phospho-Smad2 (Ser^465/467)/ Gapdh protein ratio and total Smad2/ Gapdh protein ratio in renal tissue from non-obstructed control kidney (CTR) and obstructed kidney (UUO) of Ampkα1 knockout mice (black bars, Ampkα1^-/-) and respective wild-type mice (white bars, Ampkα1^+/+) following 7 days of unilateral ureteral obstruction. *(p<0.05), **(p<0.01), ***(p<0.001) statistically significant vs. control kidney tissues of respective mice; ###(p<0.001) statistically significant vs. respective kidney tissues of wild-type mice.