Loss of Prep1 enhances cell cycling in the HSPC compartment.

<p>(<b>A</b>) Representative flow cytometric profiles showing BrdU incorporation and 7-AAD staining of LSK cells from <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> and control mice. Numbers indicate percentage of gated cells at the different cell cycle stages. Bar graphs shown on the right depict the percentages of cells in G0/G1-, S- and G2/M-phase of the cell cycle in the LSK cell population from <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> (solid bars) and control <i>Prep1</i>^<i>flfl</i> littermate (open bars) mice (mean and SD; n = 3). (<b>B</b>) Representative flow cytometric profiles showing BrdU incorporation and 7-AAD staining of Lin^- Sca1^- c-Kit⁺ (LK) cells, mainly containing myeloid progenitors, from <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> and control mice. Bar graphs on the right depict the percentages of cells in G0/G1-, S- and G2/M-phase of the cell cycle in the LK cell population from <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> (solid bars) and control <i>Prep1</i>^<i>flfl</i> littermate (open bars) mice (mean and SD; n = 3). (C) Representative flow cytometric profiles showing PyroninY and Hoechst 33342 staining of CD150⁺ CD48^- CD41^- SP cells from <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> and control mice. Bar graphs on the right depict the percentages of cells in G0 -, G1- and S/G2/M-phase of the cell cycle in the indicated cell population from <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> (solid bars) and control <i>Prep1</i>^<i>flfl</i> littermate (open bars) mice (mean and SD; n = 3).