mRNA levels of stem cells markers and EMT associated molecules in CD133⁺ and CD133^- HT29 cells.

<p>Relative mRNA expression levels of stem cell markers Oct-4 and Sox-2 (A), EMT associated transcription factors Twist and Snail (B), and EMT markers E-cadherin, fibronectin, and vimentin (C) were determined by Real-time quantitative PCR and normalized to GAPDH expression. *, <i>p<</i>0.05; **, <i>p<</i>0.01.