Loss of MMP9 expression blunts p38 responses to RSV infection.

<p>p38 activation was determined in (A) whole lung tissue and (B) bone marrow derived macrophages (BMDM) from WT and <i>Mmp9</i>^-/- mice following RSV infection, using multiplex antibodies for the phosphorylated and total forms of each protein. (C) Immunoblots (p-p38, p38 and actin) were performed on SAE cells following MMP9 silencing and RSV infection for 24 hours. Densitometry analysis was determined. Densitometry units (DU) represent pixel intensity of phosphorylated p38 as a ratio of total p38. (A) Two-way ANOVA was used to compare the time-course curves and multiple comparisons were determined by the Bonferroni method. (B-C) p values shown comparing both treatments connected by a line, determined by student t-tests.