Deletion of Dclk1 exacerbates colonic barrier dysfunction following DSS treatment.

<p><b>A</b>. Experimental plan; <b>B</b>. H&E staining demonstrated normal histology in Villin^Cre;Dclk1^f/f and Dclk1^f/f mice at baseline and confirmed a significant inflammatory response following DSS treatment (200x magnification) <b>C</b>. Western blot analysis of tight junction and adherens junction proteins demonstrated a decrease in Claudin-1, Claudin-7, and E-cadherin in the Villin^Cre;Dclk1^f/f mice after DSS treatment; <b>D.</b> FITC-Dextran levels in the serum of each mouse were determined 4 h after gavage in baseline and post DSS treatment (n = 5 for post DSS groups, *p<0.02, **** p<0.0001 Villin^Cre;Dclk1^f/f vs Dclk1^f/f); <b>E</b>. Fold change of FITC-Dextran levels in the Villin^Cre;Dclk1^f/f mice post DSS treatment.