Characterization of intestinal epithelial deletion of Dclk1 under normal conditions.

<p><b>A.</b> There is no detectable Dclk1 in the colonic epithelia of Villin^Cre;Dclk1^f/f mice. Colonic tissues stained with anti-Dclk1 antibody (Brown) under normal conditions (200x magnification). <b>B</b>. Dclk1 mRNA levels were dramatically decreased in the Villin^Cre;Dclk1^f/f mice; <b>C</b>. The expression levels of Dclk1 and TJPs in Dclk1^f/f and Villin^Cre;Dclk1^f/f mice; <b>D</b>. Intestinal mucosal integrity was impaired in the Villin^Cre;Dclk1^f/f mice. FITC-Dextran level in the serum of each mouse was determined 4 h after gavage (n = 3 for each group, *p<0.02).