Growth diversity of glioma cell line-derived GSs in the tumor neurosphere culture.

<p>We seeded glioma cell line-derived GSs in methylcellulose-containing growth medium at an initial clonal density of 10,000 cells/ 5 ml. (A) A schematic diagram of tumor neurosphere formation from a single cell clone. (B) U87 derived GS clones in the tumor neurosphere culture. A representative picture and schematic diagram of the population at day 1 (left) and 7 (right). Scale bar = 200μm. (C) Diversity for growth in the population of U87 (circles and the regression line in black); U251 (in red); A172 (in blue); T98G (in orange) derived GS clones. The frequency of clones of different size (number of cells per clone) at day 7 in a GS population (C) is shown.