IL-22 does not alter the immune response during <i>L</i>. <i>major</i> infection.

<p>Wild-type and <i>Il22</i>^-/- mice were intradermally infected with 2 x10⁶<i>L</i>. <i>major</i> promastigote metacyclics and cells from 5 week old lesions were collected and analyzed by flow cytometry. <b>(A)</b> Representative dot plots and bar graphs depict frequencies and total cell numbers of CD4+, CD8+, CD11b+, and LY6G+ cells. <b>(B-C)</b> RNA was isolated from the lesions of wild-type mice infected with <i>L</i>. <i>major</i> to assess gene expression. Data are represented as relative expression to housekeeping gene <i>rps11</i> and are representative of at least 3 independent experiments, with 3–5 mice per group. Error bars indicate mean ± SEM, *p < 0.05,*p < 0.01, ***p < 0.001.