Effects of LPS on SDF-1-induced RF/6A cell tube formation.

<p>Networks of tube-like structures were measured in a blinded manner. Representative images of tube-like structures are given: untreated (<b>A</b>); SDF-1 (100 ng/ml) alone in the lower chamber (<b>B</b>); LPS (1 μg/ml) pretreatment plus CXCL12 (100 ng/ml) in the lower chamber (<b>C</b>); combined LPS and U0126 pretreatment plus SDF-1 in the lower chamber (<b>D</b>); combined LPS and BAY 11–7082 pretreatment plus SDF-1 in the lower chamber (<b>E</b>). (<b>F</b>) Quantitative analysis of the number of tubules, expressed as the mean ± SD. Statistical significance determined using one-way ANOVA with *P < 0.05, **P < 0.01.